HeLa Cell Kit for HR-DNA Repair: MOST POPULAR

Our Most Popular HR Kit

Figures 1 and 2 and the linked Video show an overview and background of this Kit. 

This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, pathway, signaling or response genes) that affect or impact the process of Homology Repair (HR). The kit uses GFP as an in vivo readout for the HR pathway. The HeLa cells included in this Kit contain stably integrated GFP plasmid which is mutated and therefore GFP minus. Following HR repair, the GFP gene converts to wild type GFP and the cells fluoresce.   HR is initiated by transfection with an I-Sce1 Expression plasmid (included).  Analysis of pooled transfected cells reveals GFP+ cells in 2-3d post-transfection. GFP+ cells have successfully completed HR and can be tracked with single cell resolution. 

Who Should Buy This Kit?

Since HR repair pathways tend to be highly conserved from one cell to the next, we offer a ready-made HeLa cell kit to allow you to quickly perform experiments in cells that likely represent DNA repair broadly. 

R&D Labs that are interested in screening for agents, drugs, genes or gene products that specifically target the entire HR DNA repair pathway will find this kit very useful. It is well established that the DNA repair pathway is replete with druggable targets. Agents that target DBNA repair are usually good anti-cancer or anti-viral drugs.  Also, identifying other genes and gene products that interact with DNA repair is important in order to find new drug targets.  One major advantage of this kit is that it is operating in the context of a live/living cell. This means that the results will be highly physiological because events are transpiring within the cell in real time.  In addition, because it is florescence-based, it is possible to use single cell imaging to track individual cells that undergo repair and also to track the descendants of those cells using time lapse imaging.

Kit Contents  

1. HR-HeLa Cells frozen (cyro-protective medium) with the endogenous HR GFP reporter stably integrated. Cells may be sent frozen or ambient.
2. I-Sce1 gene expression plasmid (50ug).
3. Puromycin Stock.
4. A detailed protocol describing how to set up and use this kit for assaying HR mechanisms in the cell host provided.
5. Customer should specify the Kit size (25, 50, 100 assays corresponding to 50, 100 or 250 ug of I-Sce1 plasmid DNA, respectively).
6. Optional:  add WT GFP (e-GFP) as a control plasmid to this kit (50ug).