Human Neuronal Cell Based Kit for Interrogating HR-DNA Repair using I-Sce1 Site Cleavage

This is a cell-based reporter kit designed to study HR repair in a human neuroblastoma line (SH-SY-5Y) allowing the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. The kit uses GFP as an in vivo readout for the HR pathway and a plasmid expression vector containing the mega-endonuclease I-Sce1.

Who Should Buy This Kit?

R&D Labs that are interested in screening for agents, drugs, genes or gene products that specifically target the entire HR DNA repair pathway will find this kit very useful. It is well established that the DNA repair pathway is replete with 'druggable' targets. Agents that target DNA repair are usually good anti-cancer or anti-viral drugs.  Also, identifying other genes and gene products that interact with DNA repair is important in order to find new drug targets.  One major advantage of this kit is that it is operating in the context of a live/living cell. This means that the results will be highly physiological because events are transpiring within the cell in real time.  In addition, because it is florescence-based, it is possible to use single cell imaging to track individual cells that undergo repair and also to track the descendants of those cells using time lapse imaging.

Kit Contents  

1. Human neuronal cell line ( SH-SY-5Y) frozen (cyro-protective medium) with the endogenous HR GFP reporter stably integrated. Cells may be sent frozen or ambient.
2. I-Sce1 gene expression plasmid (25, 50 or 100 assay kits should be selected when ordering corresponding to 50ug, 100ug or 250ug of I-Sce1 plasmid DNA).
3. Puromycin Stock.
4. A detailed protocol describing how to set up and use this kit for assaying HR mechanisms in the cell host provided.